POSTDOCTORAL ASSOCIATE

School of Medicine

Established in 1930, Duke University School of Medicine is the youngest of the nation's top medical schools. Ranked sixth among medical schools in the nation, the School takes pride in being an inclusive community of outstanding learners, investigators, clinicians, and staff where interdisciplinary collaboration is embraced and great ideas accelerate translation of fundamental scientific discoveries to improve human health locally and around the globe. Composed of more than 2,600 faculty physicians and researchers, nearly 2,000 students, and more than 6,200 staff, the Duke University School of Medicine along with the Duke University School of Nursing, and Duke University Health System comprise Duke Health, a world-class academic medical center. The Health System encompasses Duke University Hospital, Duke Regional Hospital, Duke Raleigh Hospital, Duke Health Integrated Practice, Duke Primary Care, Duke Home Care and Hospice, Duke Health and Wellness, and multiple affiliations.

Be You.

Duke University's Department of Obstetrics and Gynecology has an opening as a Postdoctoral Associate in Dr. Danny Schust's lab supporting research on preeclampsia and requires an individual to run ongoing and planned experiments. This position ison-site.

Projects include:

* Establishment of a human stem-cell derived endometrial organoid model for use in co-culture to study pre-eclampsia and related peri-implantation disorders

* Expansion of a human preeclampsia trophoblast stem cell biobank for use in co-culture with immune and endometrial models

* Training of undergraduates and fellows in the lab who are working on the above two projects

* Assist in establishing a hypoxia-induced pre-eclampsia mouse model

Minimum Degree Requirements: Ph.D. or equivalent doctorate (e.g. Sc.D., M.D., D.V.M.). Should be used for post-doctoral fellows if the candidate has completed all of the requirements for a degree, but the degree has not been formally conferred; the candidate may present evidence of completion of the degree requirements, together with a statement documenting the date on which the degree is to be conferred.

Other Credential/Experiential Requirements: The incumbent must be a true post-doctoral fellow registered with the Post Doc Office and per the following requirements:

* The term of this appointment is limited (5 years with a possible extension for an additional year)

* The appointment involves substantially full-time research or scholarship, which may include teaching responsibilities.

* The appointment is viewed as preparatory for a full time academic or research career.

* The appointment is not part of a clinical research training program, unless research training under the supervision of a senior mentor is a primary purpose of the appointment.

* The appointee works under the supervision of a scholar or a department at Duke University.

* The appointee has the freedom, and is expected to publish the results of his or her research or scholarship during the period of the appointment.

Be Bold.

* Maintain laboratory and associated equipment in a clean, well-ordered, and functional manner. Keep supplies on hand with a well-planned inventory and ordering system.

* 2D cell culture and 3D stem cell culture, human peripheral blood mononuclear cell (PBMC) isolation, endometrium/trophoblast model

* co-culture, likely in the presence of immune or vascular cells

* Prepare cell culture samples for immunofluorescence staining (IF) or immunohistochemistry staining (IHC) including embedding samples for paraffin sections, staining fixed samples with antibodies and imaging using light, fluorescence and confocal microscopes. Analyze images using image analysis software, obtain data for publication.

* Prepare protein for western blots and analyze data.

* Design and perform molecular assays including but not limited to DNA extraction, RNA extraction, PCR, qPCR and prepare single cell for single cell RNA sequencing testing and analyses.

* Prepare, present and discuss data at lab meetings and journal clubs.

* Assist in figure preparation for manuscripts and presentations

* Maintain a scientific record of any experimental plans and processes performed

* Perform other related duties incidental to the work described herein.

Choose Duke.

Join our award-winning team as identified by Forbes magazine as America's Best Large Employer 2024 and be part of an inclusive culture that values excellence, innovation, and discovery. As an organization, we have exciting opportunities to be forward-thinking leaders in our field. We want talented individuals to join us, examine our current operations, and create innovative solutions that will revolutionize and enhance the way we approach our work.

Duke University is consistently ranked among the top universities worldwide and is renowned for its cutting-edge research across disciplines.

Beyond the engaging work, you'll also benefit from Duke's competitive benefits package including health insurance plans, generous paid time off, retirement programs with employer contributions, tuition assistance for employees and their children, and more.

Duke is an Equal Opportunity Employer committed to providing employment opportunity without regard to an individual's age, color, disability, gender, gender expression, gender identity, genetic information, national origin, race, religion, sex (including pregnancy and pregnancy related conditions), sexual orientation or military status.

Duke aspires to create a community built on collaboration, innovation, creativity, and belonging. Our collective success depends onthe robust exchange of ideas-an exchange that is best when the rich diversity of our perspectives, backgrounds, and experiences flourishes. To achieve this exchange, it is essential that all members of the community feel secure and welcome, that the contributions of all individuals are respected, and that all voices are heard. All members of our community have a responsibility to uphold these values.

Essential Physical Job Functions: Certain jobs at Duke University and Duke University Health System may include essential job functions that require specific physical and/or mental abilities. Additional information and provision for requests for reasonable accommodation will be provided by each hiring department.